Experimental evolution of protein function from random sequences

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences.

Sets of genes improved by directed evolution can be recombined in vitro to produce further improvements in protein function. Recombination is particularly useful when improved sequences are available; costs of generating such sequences, however, must be weighed against the costs of further evolution by sequential random mutagenesis. Four genes encoding para-nitrobenzyl (pNB) esterase variants e...

Protein structure and function from sequences

Detecting protein function and protein-protein interactions from genome sequences.

A computational method is proposed for inferring protein interactions from genome sequences on the basis of the observation that some pairs of interacting proteins have homologs in another organism fused into a single protein chain. Searching sequences from many genomes revealed 6809 such putative protein-protein interactions in Escherichia coli and 45,502 in yeast. Many members of these pairs ...

FURTHER RESULTS OF CONVERGENCE OF UNCERTAIN RANDOM SEQUENCES

Convergence is an issue being widely concerned about. Thus, in this paper, we mainly put forward two types of concepts of convergence in mean and convergence in distribution for the sequence of uncertain random variables. Then some of theorems are proved to show the relations among the three convergence concepts that are convergence in mean, convergence in measure and convergence in distributio...

Binary Random Sequences Obtained From Decimal Sequences

D sequences [1-15] are perhaps the simplest family of random sequences that subsumes other families such as shift register sequences [16]. In their ordinary form, d sequences are not computationally complex [2], but they can be used in a recursive form [8] that is much stronger from a complexity point of view. The basic method of the generating the binary d-sequences is given in [1]. The autoco...